Standardization of sperm management for laboratory assessment of sperm quality and in vitro fertilization in Eurasian perch (Perca fluviatilis)

Abstract Sperm handling procedures and its usage for in vitro fertilization are crucial standardized experimental operations on evaluation of reproductive performance, gamete quality, optimization protocols. In our study, the changes perch sperm motility parameters within 6-h chilled storage using 4 different activating solutions were compared. Eight sperm-to-egg ratio was also compared during fertilization. activated with modified solution cyprinids (78±11%), common (68±16%), Lahnsteiner (75±16%), Woynárovich (76±13%) showed similar progressive at 10 s after activation. At 30 activation, decreased below 5%, regardless used. Progressive significantly already 2 h (51±19%) comparison 0 (78±5%). The highest average rate (using solution) observed a 2.5×10 5 :1 (80±9%), where smallest variability values recorded (coefficient variation: 11%). However, no significant difference detected among 8 groups. According to findings, undiluted fresh is recommended use 1 post-stripping. Modified Lahnsteiner’s can be applied efficiently quality assessment applicable Eurasian perch. A allows achieve high rate; however, finding needed tested hatchery level (higher number eggs).